1. Introduction
The capacity of cells to exert forces on their environment and alter their shape as they move [3] is essential to many biological processes including the cellular immune response to infections [25], embryonic development [48], wound healing [8] and tumor growth [16]. Detecting cell shape and their changes over time as cells navigate the microenvironment are essential for understanding the multiple mechanisms guiding and regulating cell motility [74]. We propose an end-to-end pipeline for accurate cell detection, segmentation and tracking as shown in Figure 1.