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Digital imaging fluorescence microscopy: analytical purpose and low light level intensity | IEEE Conference Publication | IEEE Xplore

Digital imaging fluorescence microscopy: analytical purpose and low light level intensity


Abstract:

It is shown that the problems encountered in the use of numerical analysis on digitized images obtained from low-light-level fluorescence imaging using intensified TV cam...Show More

First Page of the Article

Abstract:

It is shown that the problems encountered in the use of numerical analysis on digitized images obtained from low-light-level fluorescence imaging using intensified TV cameras to quantify fluorescence intensities can be overcome by an appropriate treatment of the digitized image. A protocol that corrects for the dark signal and pixel-to-pixel response variations, which are the sources of the problem, is presented. The efficiency of the protocol is exemplified by the evaluation of nuclear fluorescence intensities of 300 3T3 fibroblasts of the same population sample.<>
Date of Conference: 09-12 November 1989
Date Added to IEEE Xplore: 06 August 2002
Conference Location: Seattle, WA, USA

First Page of the Article


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