Multi-Layer Micro-Nanofluidic Device for Isolation and Capture of Extracellular Vesicles Derived From Liposarcoma Cell Conditioned Media

We report on isolation, capture, and subsequent elution for analysis of extracellular vesicles derived from human liposarcoma cell conditioned media, using a multi-layer micro-nanofluidic device operated with tangential flow separation. Our device integrates size-based separation followed by immunoaffinity-based capture of extracellular vesicles in the same device. For liposarcomas, this is the first report on isolating, capturing, and then eluting the extracellular vesicles using a micro-nanofluidic device. The results show a significantly higher yield of the eluted extracellular vesicles (~84%) compared to the current methods of ultracentrifugation (~6%) and ExoQuick-based separations (~16%). [2020-0155].

I. Introduction

Liposarcoma (LPS) is the most common soft tissue sarcoma and accounts for approximately 16% of all the sarcomas [1]. Current methods for diagnosis rely on invasive tissue biopsies as standalone procedures or in combination with imaging approaches to identify tumor mass and location [2]. One of the emerging molecular targets for clinicians is the evaluation of the MDM2 DNA sequence copy numbers to distinguish between dedifferentiated liposarcoma (DDLPS) and benign lipoma [3], [4].