The Correlation Between the Cytotoxicity of Cold Atmospheric Plasma and the Extracellular H2O2-Scavenging Rate

Cold atmospheric plasma (CAP) has shown its promising capability in cancer treatment both in vitro and in vivo. However, the anti-cancer mechanism is still largely unknown. CAP may kill cancer cells via triggering the rise of intracellular reactive oxygen species, DNA damage, mitochondrial damage, or cellular membrane damage. While the specific vulnerability of cancer cells to CAP has been observed, the underlying mechanism of such cell-based specific vulnerability to CAP is completely unknown. Here, through the comparison of CAP treatment and H₂O₂ treatment on ten different cancer cell lines in vitro, we observed that the H₂O₂ consumption rate by cancer cells was strongly correlated to the cytotoxicity of CAP treatment on cancer cells. Cancer cells that clear extracellular H₂O₂ more quickly are more resistant to the cytotoxicity of CAP treatment. This finding strongly indicates that the anti-oxidant system in cancer cells play a key role in the specific vulnerability of cancer cells to CAP treatment in vitro.