Abstract:
Fluorescence microscopy is a powerful tool for the rapid identification of target organisms. However, natural autofluorescence often interferes with identification. Time-...Show MoreMetadata
Abstract:
Fluorescence microscopy is a powerful tool for the rapid identification of target organisms. However, natural autofluorescence often interferes with identification. Time-gated luminescence microscopy (TGLM) uses luminescent labels with long persistence in conjunction with digital imaging to regain discriminative power. Following the excitation pulse, short-lived autofluorescence decays rapidly whereas the long-lived emission from lanthanide doped polymer beads persists for hundreds of microseconds. After a short resolving period, a gated high gain camera captures the persistent emission in the absence of short-lived fluorescence. We report on the development of a TGLM software system for automated scanning of microscope slides, and show its use to resolve luminescent microspheres within a matrix of autofluorescent algae.
Date of Conference: 01-03 December 2009
Date Added to IEEE Xplore: 19 January 2010
ISBN Information:
Faculty of Science, Macquarie University, Sydney, Australia
Faculty of Science, Macquarie University, Sydney, Australia
Faculty of Science, Macquarie University, Sydney, Australia
Faculty of Science, Macquarie University, Sydney, Australia
Faculty of Science, Macquarie University, Sydney, Australia
Centre of Infectious Diseases and Microbiology, University of Sydney/Westmead Hospital, Sydney, Australia
Faculty of Science, Macquarie University, Sydney, Australia
Faculty of Science, Macquarie University, Sydney, Australia
Faculty of Science, Macquarie University, Sydney, Australia
Faculty of Science, Macquarie University, Sydney, Australia
Faculty of Science, Macquarie University, Sydney, Australia
Centre of Infectious Diseases and Microbiology, University of Sydney/Westmead Hospital, Sydney, Australia