Polarized fluorescense of alkyl derivatives of fluorescein, MitoFluo and C8-Fl, in solutions with liposomes

We present the study of interaction of fluorescein derivatives, MitoFluo and C
₈
-Fl with two types of liposomes modeling cellular and mitochondrial membranes. Polarization anisotropy, fluorescence lifetimes, and rotational diffusion times have been determined from time-resolved polarized fluorescence experiments. The analysis of the experimental results has shown that both fluorescein derivatives interact with liposomes and that MitoFluo embeds in liposomes more effectively than C
₈
-Fl.