Dual-wavelength fluorescence imaging for photodynamic therapy planning and monitoring

Increase in photodynamic therapy (PDT) efficiency requires both proper planning with the evaluation of photosensitizer (PS) distribution within the tissue and procedure monitoring. We report on perspectives of dual-wavelength fluorescence imaging (FI) at 405 nm and 660 nm for PDT procedure assistance aimed at evaluation of chlorin-based photosensitizer accumulation depth for both topical administration and intravenous injection. The study includes Monte Carlo simulations, experiments on tissue phantoms and laboratory animals, and clinical monitoring.