Haemocompatibility test of simple Magnetic Nanoparticles using the distribution of deformed RBCs

Haemocompatibility of nanoparticles is usually evaluated by hemolysis. However, hemolysis assessment does not measure the dysfunctional erythrocytes with pathological changes on the unbroken cellular membrane. This paper presents a microfluidic tool based on a constriction channel with a hyperbolic-shaped contraction capable of evaluating the biocompatibility and toxicity impact of magnetic nanoparticles (MNPs). The aim of this study is to evaluate the deformation index of red blood cells (RBCs) that have different concentrations of MNPs and their distribution on the microchannel. The results showed that MNPs affected the deformability of RBCs, as long as the MNPs concentration increases, however with the increase of the temperature, that dependence between MNP concentration and deformability decreases.