All-in-One Optofluidic Platform for Differential Diagnostics of Multiple Biomarkers with Single Molecule Sensitivity

Amplification-free and high throughput single nucleic acid detection, with minimal user input, is achieved by integrating optical waveguides with programmable valve array on a single microfluidic platform. Automated preparation and analysis of a dual protein-nucleic acid assay for Zika viral diagnostics is demonstrated.