Comparison of detection efficiency of real-time polymerase chain reaction chip for fluorescence detection by different types of coating materials

In this paper, we used a PCB substrate that used a white silk legend printed on a heater pattern in the process of detecting fluorescence with an RT-PCR chip fabricated on a PCB substrate. However, since the fluorescence brightness deviations between chips were large, accurate quantitative analysis was difficult. Therefore, we fabricated chips using 4 kinds of PCBs that can be coated on the PCB substrate and experimented to select the optimal chip for fluorescence detection and obtained the results.