Nanoscale imaging by using label free microscopy techniques

The objective of our work is connected with label free investigations at nanoscale by using a new multimodal microscopy system by using near field label free techniques. The system includes the techniques with hundred nanometers resolution and with few nanometers resolution, being able to image simultaneously the same sample area giving complementary information. Our system includes also an atomic force microscope. To image the human skin samples has been used two label free techniques working at nanoscale: scattering scanning near optical microscopy(s-SNOM) and second harmonic generation using an apertureless-scanning near optical microscope (SHG-SNOM).