The anodic aluminum oxide (AAO) template fabricated Localized Surface Plasmon Resonance (LSPR) sensor for the plasmonic coupling effect study

The localized surface plasmon resonance (LSPR) sensing has drawn significant attention in biosensing fields due to its label-free, dynamic, sensitive, and ease-of-operation sensing capability. In our study, we utilized an anodic aluminum oxide (AAO) template to fabricate a large-area LSPR sensor with adjustable nanostructure parameters such as period and diameter. By using different nanostructure patterns, we investigated the plasmonic coupling effect and optimized the nanostructure geometry. Our research demonstrates the real-time and multipoint detection of pathogen DNA sequences. Based on the above features, we believe that this platform has the potential for point-of-care (POC) biosensing applications.