Integrated magneto-electrochemical sensing arrays for multi-parametric screening of Alzheimer’s disease related biomarkers

Multi-parametric screening of Alzheimer’s disease (AD) related markers at early stage is urgent for AD clinical treatment to prevent its progressive deterioration. This study introduces an integrated magneto-electrochemical sensing arrays (MeSA) platform. It consists of disposable electrochemical sensing arrays, a magnet holder, and a pocket-size read-out circuit that can facilitate Bluetooth communication. The screen-printed electrode (SPE) arrays have 6 channels and can be used for analyzing multiple biomarkers simultaneously. Using the MeSA platform, we have investigated the Alzheimer’s disease (AD) related biomarkers, Amyloid β (Aβ)1-42 and lactoferrin (Lf). During signal detection, the chronoamperometry response is generated rapidly within 1 min via enzymatic reaction. The MeSA platform detects Aβ1-42 with a limit of detection (LOD) <0.1 pg/ml and Lf <1 pg/ml, which outperforms the commercial enzyme-linked immunosorbent assays (ELISA) as it is >10 times more sensitive. We believe that MeSA platform holds immense potential for point-of-care testing in early-stage AD due to its capability to detect multiple biomarkers, compact size, cost-effectiveness, and rapid detection time.