Distinguishing between dsDNA and DNA with a single-base mismatch using solid-state nanopores

Nucleic acid markers of the same family show single nucleotide polymorphisms (SNP) in studies where SNP are associated with biological features of cancer. This work proposes a rapid and simple method for detecting single-base mismatch DNA by binding through identical probe DNA and using solid-state nanopores with small pore sizes for differential recognition of dsDNA versus single-base mismatch DNA. This is reflected in the blocking current and translocation speed. The strategy proposed in this work is efficient and capable of accurately identifying SNP in cure mutants. This is potentially relevant in early disease diagnosis and prevention.