Immunoagglutination Diagnosis with Acoustofluidic Technology

Immunoagglutination assays rely on the detection of the chemical interaction between antibodies (Ab) and antigens (Ag), which occurs through non-covalent bonds. Agglutination refers to the formation of macroscopic aggregates of nanoparticles. In this method, the antigen or antibody is immobilized on the surface of inert particles. Our goal is to introduce an acoustofluidic chip that catalyzes the Ag-Ab reaction. Acoustofluidic technology utilizes ultrasonic forces within microfluidic systems. Here, we present the results of the acoustofluidic-enhanced agglutination test to detect C-reactive protein (CRP) and antibodies against streptolysin O toxin (ASO). Our findings demonstrate an eight-fold increase compared to conventional tests.