Identification of Candida Krusei by 18S rRNA Gene and Investigation of SAP1 Gene in Samples Isolated from Female Genital Tract Infection

This study aims to develop an 18SrRNA-based technique for Candida krusie identification and virulence gene detection (SAP1), Between September 9, 2020 and June 30, 2021, this research was conducted at the Maternity and Children's Hospital in Nasiriyah, Thi-Qar province. Vulvovaginal candidiasis (VVC) was detected in 150 randomly selected women between the ages of 17 and 50. C. krusei patient isolates were recovered from individuals with Vulvovaginal. Candida albicans was the most frequently detected species among those examined (68.57%), followed by Candida krusie (11.43%), Candida glabrata (8.57%), Candida parapsilosis (5.71%), and Candida tropicalis (5.71%). All of the samples had the 18S rRNA gene, as determined by testing. Through sequencing and phylogenetic analysis, genetic distinctions between local isolates and global strains were uncovered. Seven of 10 (87.5%) isolates were SAP1-positive. 18S rRNA gene is 100% accurate in identifying C. krusie; nonetheless, the presence of this virulence gene may play a significant role in the occurrence of infection.