Label-free method for screening antibodies against thyroxin

The methods for fast selection of the antibodies that provide the maximal performance of assays are highly demanded. Here, a label-free optical method is presented for screening antibodies against free thyroxine (fT4). The method features real-time monitoring of changes in the biolayer thickness on a sensor chip caused by association and dissociation of molecules. The single-used sensor chip is a commonly available microscope glass cover slip. Among others, the benefits of the developed method include controllable in real time immobilization of virtually any biotinylated antigen/antibody on universal biotinylated chips due to the used biotin-streptavidin bond.