Effect of AgNPs Biologically and Chemically Synthesized on Phanerochaete Chrysosporium Antioxidant Enzymes Activities

The silver nanoparticles (AgNPs) were synthesized by both bio- and chemical-based approaches in which celandine (Chelidonium majus) aqueous extract and trisodium citrate were used as reducing agents and silver nitrate (AgNO3) as metal precursor, respectively. The formation of AgNPs was confirmed by color change and absorption spectroscopy. The antioxidant response of Phanerochaete chrysosporium, following exposure to various concentrations of biologically and chemically synthesized AgNPs and depending on the mycelium age (7 and 14 days), was further studied. Results revealed that the effect of AgNPs synthesized by biological reduction determined an increase of antioxidant enzymes activities (catalase and superoxide dismutase) with the increase of nanoparticles concentrations. Both, biological and chemical reduction revealed a very similar CAT activity at both intervals studied and at almost the same scale value in all P. chrysosporium variants.