Testing and characterization of a biosensor for the detection of infections in tissue based on Rh6G and AuNP functionalized with BSA

In this study we present a microfluidic device as an electrochemical biosensor with a role for detecting fungal skin/tissue infections. This biosensor, made by colloidal aqueous solution of rhodamine Rh6G - gold nanoparticles (AuNP) functionalized with bovine serum albumin (BSA) with applications in selective detection and identification of infections in the human body.The interaction between the prepared Rh6G-Au NPs and BSA samples were characterized morphologically with Scanning Electron Microscopy-SEM, electrically and by optical florescence microscopy. Gold nanoparticles (AuNPs) have been used as carriers of Rh6G and BSA in order to obtain an amplification of the electrical signal in the biosensor with the role of detecting tissue infections and also in transporting drugs to the affected tissues in the body. Furthermore, we used also COMSOL software to enables the simulation of realized designs for the associated fluidic microchannel, an important component part of the biosensor. We hope by improving and developing the propsed model to progress in the area of clinical diagnostics and to answer some challenges about protein with high binding affinity and highest energy acceptance efficiency