Automation for two-position fluorescence measurement of a single cell captured in a microfluidic biochip

Two-position measurement for fluorescence of a single cell captured in a microfluidic chip has been conducted manually. This paper describes the automation accomplished on moving the chip back and forth at two definite positions by a robotic arm controlled with a computer using Python codes. A single lung cancer cell was captured in the chip; measurements at two positions (cell and background) were automated to demonstrate the signal enhancement due to more accumulated reagents.