Fast holographic scattering compensation for deep tissue biological imaging

Scattering in biological tissues is a major barrier for in vivo optical imaging. The resulting distortion can be corrected by shaping the excitation wavefront to redirect power into a single point in the imaging plane, but finding the optimal correction pattern is nontrivial. Several strategies have been developed to this end, including indirect wavefront sensing techniques which aim to redirect light from a subset of the scattered modes into the focus using an iterative approach. Two indirect wavefront sensing techniques, termed IMPACT and F-SHARP, have recently enabled impressive in-vivo imaging results in various tissues including the mouse brain [1] , [2] . However, further development is required to increase the imaging depth and match the persistence times of many living tissues.