A continuous cell culture system with automatic OD measurement and medium addition

In order to realize the automated operation of continuous cell culture to reduce the work intensity of researchers and establish the steady-state growth of cells, this paper designed a continuous cell culture system using LED light source, photoelectric sensor, liquid level sensor and STM32 processor, it can automatically measure OD and control the addition of fresh medium. In this system, photoelectric sensor is used to receive the light sent by LED light source through the cell solution, STM32 processor is used to process the signal to get the OD value of the cell solution. The control terminal controls the operation of charging pump and discharging pump respectively according to the OD value and the feedback signal of the liquid level sensor, so as to establish the automatic operation of continuous cell culture, replace the manual operation by researchers, and avoid the risk of contamination during manual operation. The experimental results show that the system is accurate in OD measurement and has high reliability.