FPGA-Assisted Nonparallel Impedance Cytometry as Location Sensor of Single Particle

Microfluidic impedance cytometry, as an established method for single cell analysis, has a strong sensitivity for the position of cells or particles within the detection area. The location dependency of single beads/cells are represented by the amplitude of output current signals, but such a parameter is also affected by either sizes or vertical locations of target projects. In this work, we present a nonparallel impedance cytometry to settle this issue and apply a field programmable gate array (FPGA) to design a detection system to process signals from impedance electrodes. With a new layout of electrodes, we enhance the performance of impedance cytometry as a location sensor and provided a new feature to characterize the location of single cells/beads. The new design demonstrated its functionality on 10 µm beads. Besides, a cost-saving way to measure impedance signal is also provided. Ø