Development of a portable lens-free holographic microscope to imaging cell cultures

Microscopes are essential imaging tools for accessing micro scale information in several contexts, including routine visual analysis for cell culture. As an alternative to traditional optical microscopic instrumentation, lens-free microscopy is an emerging modality, which is efficient to provide portable apparatus that provides images with decoupling resolution from the field of view. This paper presents compact and easy-to-use imaging equipment based on optical lens-free holographic microscopy, which performs amplitude and phase images from a sequence of captured images at different distances from the sample, resulting in images with almost 30 square millimeters and 4 micrometers of resolution.