Study of Correlation between Ultrasonic Electrokinetic Polarization and Dielectric Polarization in Cell Suspensions

Ultrasonic electrokinetic polarization technique is a new method to study the charge characteristics of cell surfaces. In order to understand the influence of electrokinetic polarization on the dielectric properties of cell suspensions and characterize the electrokinetic polarization of cell suspensions by the dielectric measurement, the dielectric characterization method and related model of electrokinetic polarization are established in this paper. The relative change rate of ε
_r
and tan δ is used to characterize the electrokinetic polarization characteristics of the rabbit blood cell-phosphate buffer solution suspension with the different cell concentrations. The experimental results show that at the temperature of 37 °C and the electrical field frequency of 1 kHz, the electrokinetic polarization generated by the ultrasonic wave of 1.7 MHz, 2.76 MPa, will increase the ε
_r
and decrease the tan δ of the cell suspension. With the increase of the concentration of the blood cell from 4.85×10
⁴
to 4.85×10
⁹
ml
^-1
, the relative change rate in the rabbit blood cell suspension will firstly increase, then decrease, and then increase, and finally decrease, which is consistent with the changing trend of the electrokinetic polarization voltage of the suspension as well as the established model. The study in this paper indicates the relationships between the ultrasonic electrokinetic polarization and the dielectric polarization, which provides a theoretical and experimental basis for understanding the electrokinetic polarization characteristics and cell surface charge of cell suspensions through the dielectric measurement method.