An Integrated Microfluidic System for Early Diagnosis of Breast Cancer in Liquid Biopsy by Using Microrna and FET Biosensors

Breast cancer is one of the leading cancers among women globally with a high mortality rate if not diagnosed in early stages. Liquid biopsy could serve as a non-invasive method for early diagnosis of biomarkers (such as microRNA-195) in blood associated with breast cancer. In this study, we developed an integrated microfluidic system for detecting targeted microRNA-195 by using field-effective-transistor biosensors to quantify an extremely low concentration of microRNA after extraction of extracellular vesicles and microRNAs in plasma. The entire process could be automated with a limit of detection as low as 1 fM. It could be therefore promising for accurate and rapid diagnosis of breast cancer.