Exploring tumor-stroma interactions using combined autofluorescence and SHG imaging

We present the results of investigation of metabolic activity of cancer cells and fibroblasts and structure of collagen in 3D model in vitro and mouse tumors in vivo. The metabolism was assessed using fluorescence lifetime imaging of the metabolic cofactor NAD(P)H. Second harmonic generation (SHG) imaging was used to analyze the extent and properties of collagen.