Optically controlled design of bi-functional agents for development of competitive immunoassays of enhanced sensitivity

Label-free approach is developed based on the spectral-correlation and spectral-phase interferometric methods for characterization and fine-tuning of bi-functional ligands, which can improve the limits of detection of immunoassays by several orders of magnitude. The concept was verified by development and optimization of an ultrasensitive test system for registration of thyroxine (a marker of thyroid diseases).