Rational Design of Nanoparticle-Based Agents for Effective Targeted Drug and Gene Delivery to Eukaryotic Cells

Nanoparticles functionalized with different bioreceptors have considerable potential for use in targeted drug and gene delivery applications. Here we show a comprehensive strategy for the development of such nanoparticle-based agents. We used a number of optical techniques including confocal microscopy, scanning electron microscopy, and imaging flow cytometry to achieve agents` reliable performance in eukaryotic cell culture.