Growth Stage Prediction of Haematococcus pluvialis Based on GRNN and PNN Neural Networks

Haematococcus pluvialis is a freshwater single-cell green algae and is currently considered to be the most ideal source of natural astaxanthin. When Haematococcus pluvialis is cultured in a "two-step method", it is mainly divided into cell proliferation stage and astaxanthin accumulation stage. Due to the long culture period of Haematococcus pluvialis and many factors during the cultivation process, experimentally measured characteristic values such as cell radius have a non-linear relationship with growth status. In this paper, the characteristic values of cell growth stage were characterized by cell radius, OD value (absorbance value) and cells number. The GRNN (Generalized Regression Neural Network) and PNN (Product-based Neural Network) neural networks with strong mapping capabilities and fast learning speed to build models, growth stage was predicted by the characteristic values of the cell, and compare the two prediction models, which is convenient for researchers to classify the two different growth stages of Haematococcus pluvialis cells and provide a basis for designing cell proliferation and astaxanthin accumulation experiments.