Shape-printed nanozyme coated wet tissue paper based sensor for electrochemical sensing of 8-Hydroxy-2’ -deoxyguanosine

Flexible and biocompatible wearable sensors for the management of chronic diseases are in demand. The disposable, biocompatible, flexible and lightweight conductive paper-based sensing system was fabricated for 8-hydroxydeoxyguanosine. This biomolecule can biomark the early symptoms of various clinically important diseases such as cancer, diabetes, and atherosclerosis. Wet tissue paper modified with graphene nanoparticles was used for the analysis of 8-hydroxydeoxyguanosine in healthy human and patient biological samples. Here imprinted nanozymes were used as a receptor that promotes selective sensing of 8-hydroxydeoxyguanosine. Shape printed nanozyme contains hydroxyl and carboxyl groups which promote the penetration of 8-hydroxydeoxyguanosine and its selective adsorption. Graphene and nanozyme both catalyze the selective sensing of 8-hydroxydeoxyguanosine. When 8-OHdG was added the specific interaction of 8-OHdG and nanozyme was examined by current signals. The current signal was linear with 8-OHdG concentration. The fabricated shape printed nanozyme based sensor shows a biological justifiable sensitivity with linear range of 1.0-100.00 μg mL-1 with no matrix effects. The Shape printed nanozyme modified tissue paper could be used as a wearable patch for early monitoring of oxidative stress biomarker by cancer patients itself without any preprocessing plan.