Experimental Setup for Measurements the Hydrogen Concentration in Exhaled Rat's Air

This study focuses on methods for determining the concentration of hydrogen in the body of laboratory animals. Laboratory rats were used to estimate the amount of hydrogen in the body based on the concentration of hydrogen in the exhaled air. In the course of the experiment, ten male Wistar albino rats were used. The experimental setup was developed and tested based on an analysis of previously published works by other authors. The experiment used the classic hydrogen breath test with lactulose load. The obtained hydrogen level dependence on time in the lactulose test is consistent with the literature data. There were also some changes proposed in the experimental setup, which should improve the quality of the obtainable data. As a result, it is planned experimentally to confirm that dietary preferences can alter the composition of the intestinal microbiota in the direction of increasing hydrogen production, which can serve as the basis for the development of new therapeutic and nutritional algorithms. In the future, this work should serve as a suitable basis for the development of new methods of hydrogen therapy.