A multi-channels axon-stretch device for mechanical stress study

To mimic the nature stretch of axon, we developed a multiple channel device which can apply mechanical stretch to the neural axon. This device with four cell culture chambers and adjustable speed, which could be used to screening the stretch speed parameters and the potential nutrition factors that could be used to facilitate the regeneration of neuron fibers in vitro. The current design can range from 0.25um (min) to 50um (max) for each step, which covers the physiological and pathological stretch speed. The increased samples and the stepping ranges allow sophisticated experiments could be performed with this new device. The system compositions including structures and each module function are introduced in detail and the performance of this device is tested. The test of cell viability of this culture chamber show that the neuron cell and Schwann cells (SC) sustain normal morphology and cell viability. Axons stimulated by the mechanical stretch for 5 days at the speed of 1mm/day was tested by this system. The results showed that axon growth was promoted by mechanically stretch. The multi-channel axon-stretch device has been approved to be functionally in to culture neurons and stretch axons in one direction.