Study of Protein Water Interactions in GroEL Molecular Chaperonins using Terahertz Spectroscopy

THz spectroscopy is an attractive technique to study the interaction of proteins in water as it is highly sensitive to water mediated interactions. GroEL, a barrel shaped chaperonin known for assisting protein folding, is studied using THz-TDS technique for varying concentrations. Results show significant decrease in absorption with increasing concentrations demonstrating the formation of more bound water due to the presence of GroEL complex.