Cell Segmentation Via Region-Based Ellipse Fitting

We present a region based method for segmenting and splitting images of cells in an automatic and unsupervised manner. The detection of cell nuclei is based on the Bradley's method. False positives are automatically identified and rejected based on shape and intensity features. Additionally, the proposed method is able to automatically detect and split touching cells. To do so, we employ a variant of a region based multi-ellipse fitting method that makes use of constraints on the area of the split cells. The quantitative assessment of the proposed method has been based on two challenging public datasets. This experimental study shows that the proposed method outperforms clearly existing methods for segmenting fluorescence microscopy images.