A Study on Homocysteine Biosensors Completely Constructed on Anodized Titania Nanotubes

Homocysteine biosensors, based on titania nanotubes as a two-electrode system, were studied herein. The working electrode used was an immobilization of a D-amino acid oxidase enzyme by glutaraldehyde on titania nanotubes, and the reference electrode was a titania nanotube. The structure showed a reversible reaction in an electrochemical analysis. Similar cyclic voltammetry indicated that the sensitivity was 53.4 nA/μM, the corresponding correlation coefficient of linearity R
²
was 0.998 in a detecting range from 0 and 70 μM, and the limit of detection was about 1.5 μM.