Low-Cost Optical Transmittance Measurements for the Detection and Quantification of Agglutination in Dual-Phase Lab-in-Tubing Microfluidics

This letter reports an extremely low-cost system utilizing off-the-shelf line break sensors and a simple lab-in-tubing platform for detecting and quantifying agglutination assays. This system is capable of detecting agglutination over five orders of magnitude of analyte concentration, from 0.0128 to 5000 μg/mL. In addition, the system can determine low-level agglutination detection classification in two regions, i.e., 0.0128 to 0.32
μ
g/mL and 1.6 to 40
μ
g/mL, without using expensive microscopes, complex optical arrangements, or intensive computational requirements.