Applications of Superpixels on Phase Contrast Microscopy

Cellular microscopy is enhanced by computational paradigms such as image processing, computer vision, and machine learning. Image segmentation is vital for quantifying cell images, enabling tracking and subsequent applications. Superpixel algorithms offer a promising solution to challenges in phase contrast image segmentation, as they group pixels with similar characteristics into meaningful regions. By preserving essential structural information and reducing complexity, superpixels improve accuracy in cell-related measurements and facilitate the study of dynamic cellular processes. This paper presents a comparison of popular superpixel algorithms applied to cellular microscopy, highlighting their contributions to image analysis and interpretation in cell biology research.