Sprouting Buds of Petunia Hybrida Cuttings Under Normal Conditions in Vitro Culture

Petunia hybrida is a genus of flowering plants with beautiful colors, popularly grown in modern architecture. In Vietnam, Petunia hybrid is propagated using cuttings or grown from seeds. The application of tissue culture in agriculture produces high-quality and inexpensive plant varieties. In vitro regeneration is a modern and useful method in agriculture. Our research aims to investigate the best sterilization method for samples and the effects of sucrose concentration and coconut water ratio added into Knudson C medium (KC) for sprouting in vitro sprouts of Petunia cuttings under normal conditions. We used a combination of 70%, 75%, and 80% ethanol and 0.5%, 0.75
^%
, and 1% NaOCI to sterilize the samples and studied the effects of sucrose and coconut water added to KC medium on the development of in vitro sprouts. We found that using 70% ethanol combined with 0.75% NaOCI, and then adding 10% (g/L) sucrose and 15% (v/v) coconut water to KC medium ensured the best survival and development of in vitro Petunia hybrida sprouts under normal conditions.