Validation of a UV Spectrophotometric Method for Quantification of Omeprazole Using Different Types of Diluents

Introduction: Omeprazole treats intestinal ulcers and gastroesophageal reflux. Extemporaneous preparations are one of the primary forms of administration in hospitalized patients. Although its preparation is carried out under safe conditions, there are no optimal storage and preparation conditions to ensure its physicochemical stability. Thus, accessible analytical methods are required to guarantee quality control in hospitals. Objective: This study validated a UV spectrophotometric method for quantifying omeprazole in extemporaneous preparations. Methods: Omeprazole (10
$\text{pg}/\text{mL})$
, diluted in three different diluents, was analyzed under light and temperature conditions. The validated test performed for the system were linearity, precision, and specificity. For the method, linearity, precision, accuracy, and analytical stability, were tested. Results: The validated tests applied for the three different diluents were within the required parameters, therefore, the UV spectrophotometric method performed in this study is a valid procedure to quantify omeprazole in extemporaneous preparations. Conclusion: Validation of low-cost and accessible procedures for drug quantification are essential, ensuring quality control of extemporaneous preparations used in hospitals.