Modular Wavelength Adaptation of the Dynamic Optical MicroEnvironment

The DOME is a powerful and adaptable platform that facilitates the study of light-reactive systems at the microscale. While the projection module of the DOME can produce light patterns with high spatial and temporal resolution, the maximum irradiance (incident electromagnetic energy per unit area) that can be generated by its native LEDs is limited. Increasing the irradiance is crucial to enabling new biomedical applications such as inducing DNA damage. In this paper, we present a modular solution to allow general light sources to be used with the DOME. By switching to a high-powered near-UV light source, we show that DNA damage can be caused by the Epi-DOME's projection system at a targeted location.