Immobilization strategy of liposome modified cantilever biosensor arrays combined with microfluidic channels

Array sensors allow to determine multiple components and parameters in target solutions. In this study we combine a microcantilever sensor array, utilizing a NiCr gauge film, with PDMS microfluidic channels. PDMS microfluidic channels are formed by a 3D printed mold. The microcantilever sensors are sensitive to structural modifications in a preabsorbed layer. The effect of static flow to the sensor signal is under 2 ppm/min. In this study we demonstrate the immobilization of liposomes on the cantilever surface via the microfluidic channel and afterwards the detection of alpha-synuclein proteins interacting with the liposomes.