System integration of a non-contact ultrasonic disruptor

In recent years, ultrasonic cell disruption has been widely used in the field of bioengineering. Depending on whether the biological sample is in direct contact with the ultrasonic fragmentation equipment, there are contact fragmentation and non-contact fragmentation. The contact-type ultrasonic disruption system is simple in structure, but the sample is susceptible to external contamination. Therefore, an automatically controlled non-contact ultrasonic disruption system is designed in this work. The system uses the STM32F103RCT6 as the main control chip, which can control the signal generation and collect relevant data. At the same time, the driving circuit and impedance matching circuit are also designed, and is equipped with a serial screen as the interactive interface. Under the conditions of 50% duty cycle, 60s ultrasonic pulse period and 10 cycles of ultrasonic pulse, the system can effectively disrupt Spirulina cells and the number of fragments in the 10–20 µm and 20–30 µm ranges after fragmentation increases by 33% and 67%, respectively, compared to the number of fragments before fragmentation.