Microsphere Superlenses-Based Optical Super-Resolution Imaging of Ovarian Tissue Sections

Tissue sections can reveal tumour-specific changes, and a tumour diagnosis can be made by analyzing the arrangement and distribution of cells on the surface. Although histopathological diagnosis based on tissue section observation has been the gold standard for tumour diagnosis, there is still much room for improvement in terms of scale due to the limited spatial resolution of conventional optical microscopy. Microsphere super-resolution microscopy offers a potential solution to these problems. Super-resolution imaging of the surface of tissue sections was achieved with the aid of microspheres fixed to the surface of the tissue section and a removable piezoelectric ceramic displacement stage. A single microsphere of barium titanate, measuring
$50\mu\mathrm{m}$
in diameter, is capable of achieving super-resolution and is particularly suitable for observing the surface nanostructure of tissue sections. In our experiment, we successfully combined the microsphere super-resolution microscope with scanning stitching techniques to obtain tissue section structure images with a resolution lower than 200nm. Our research results will help improve the accuracy of tissue section observations and promote advancements in this field.