Effect of Salbutamol on Red Blood Cells by Scanning Flow Cytometry

Salbutamol serves as a means to relax smooth muscles and relieves bronchospasm. However, mechanisms of salbutamol action in blood are not clear. In particular, the influence of salbutamol on erythrocytes is far from completely understood. In this work, applying scanning flow cytometry, we observed experimentally for the first time the dynamics of anion exchange rate of transmembrane Band 3 protein of human erythrocytes in the presence of salbutamol in blood. It was found that there is the appropriate concentration of salbutamol to initiate the activation of Band 3 protein. Thus, the use of scanning flow cytometry allowed to clarify quantitatively the molecular kinetic mechanism of salbutamol action on human erythrocytes.