Application Software for Cardiovascular and Muscular Studies of Zebrafish Larvae

Zebrafish is a popular model organism for human diseases research, drug testing and other biomedical study tasks. Cardiovascular and muscular system parameters are valuable indicators of organism disorders and its response to stress. Noninvasive and productive methods for assessing zebrafish body state are rapidly evolving. In this study, we present an image processing algorithm and software application for such a method that enables simultaneous imaging and quantification of the cardiovascular and muscular systems of optically transparent biological objects. We have described the algorithm key steps and experimentally demonstrated its effectiveness on zebrafish larvae.