Automated Method for Calibration Findings of Auto Slide Scanner Using Automated Digital Microscopy

Applications in the automated microscopy require careful calibration of the microscope system. In one such scenario where automated microscope is made to work with auto slide scanner, accurate measurement of the calibration parameters becomes an essential procedure. Here, we propose an automated method for finding the calibration parameters of a microscope based auto slide scanner. Microscopic images of a grid micrometer slide are analyzed for finding calibration parameters. Apart from the automated microscope and auto slide scanner, the procedure requires a digital camera and a grid micrometer slide. This calibration finding embraces the following parameters of camera and slide scanner: (a) measuring the pixel size in micrometers, (b) measuring the camera orientation and (c) measuring the linearity and uniformity of the slide scanner. The proposed method is accurate, reproducible and scalable enough to be suitable for applications requiring in-situ calibration.