CRISPR Cas9 genome editing approach for gRNA of the genes associated with Schizophrenia: A computational approach

It is of critical need to increase the number of restorative and curative approaches against Schizophrenia. In our work, focus has been made on identifying the best guide RNA (gRNA) for one such approach namely Crispr Cas9 system. These gRNAs directly target the major nine genes responsible for Schizophrenia in Crispr Cas9 technique. To accurately identify said gRNAs for our study, various computational tools and servers working on different algorithms have been used along with off and on target checks. Crispr is an emerging and commendatory gene editing technology and gRNAs play a key role in the technique, hence it becomes possible for us to better understand the cause and the pathophysiology behind the disease on a genetic level through the use of gRNAs. Further validation of the results through laboratory protocols and clinical criteria presents a novel approach in the prevention and treatment of the disease.