Effect of a composition based on aluminosilicon particles and alcoholic chaga extract on human mesenchymal stem cells

Inonotus obliquus, also known as chaga, is a parasitic fungus growing on birches and used in traditional medicine to treat various health problems. The effect of immobilized on aluminosilicon particles alcoholic extract of chaga and liquid alcoholic extract of chaga were tested on human bone marrow stem cells. NADPH-dependent oxidoreductase activity was assessed by MTT test. Nitric oxide production was measured with Griess reagent. Apoptosis/necrosis was evaluated by using vital staining with acridine orange and ethidium bromide. Immobilized bioactive compounds from chaga significantly decreased NADPH-dependent oxidoreductase activity in MSCs. While liquid alcoholic extract of chaga have no have any influence on NADPH-dependent oxidoreductase activity in MSCs. Immobilized chaga induced increased nitric oxide production, but liquid extract of chaga decreased nitric oxide production by MSCs. All types of chaga extract significantly augmented apoptosis/necrosis of MSCs compared with control. Obtained data indicates cytotoxic effect of chaga extract.