LED light source for fluorescence endoscopy using quantum dots

We present an LED light source for use with standard clinical endoscopes to enable visualization of tissues labeled with quantum dots (QDs). QD-assisted endoscopy may improve the outcome of surgical endoscopic procedures by identifying specific tissue types. QDs offer several advantages over current fluorescent stains due to their high target selectivity, long-lasting fluorescence, large excitation and narrow emission bands, and multiplexing capabilities. The prototype presented is compact, modular in design, and was built at low cost making it competitive with commercially available light sources. The device's efficiency is evaluated by measuring light intensity at discreet locations and by successfully illuminating a chicken tissue sample non-specifically labeled with a 250nM or 500nM QD solution. Ultimately, this device serves as a step towards incorporating QDs into real time, image-guided surgical procedures.